产品货号：
GS4829
中文名称：
重组人TPO
英文名称：
Recombinant Human Thrombopoietin
产品规格：
2μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 80kDa,consisting of a 332 amino acid residue with a predicted molecular mass of approximately 35 kDa.As a result of glycosylation,the recombinant protein migrates with an apparent molecular mass of 80±10 kDa in SDS-PAGE.
储存条件：冷冻（-20℃）
概述
Thrombopoietin(TPO),the ligand for the receptor encoded by the c-Mpl proto-oncogene,is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo.The cDNAs for TPO have recently been cloned from canine,murine and human sources.The proteins from these three species are highly conserved,exhibiting from 69 - 75%sequence identity at the amino acid level.Two distinct domains,separated by a pair of arginine residues that may be a proteolytic cleavage site,have been identified in TPO:the amino terminal region exhibiting sequence homology to erythropoietin and the carboxy terminal region containing multiple potential N-linked glycosylation sites.Recombinant TPO has now been shown to stimulate the maturation,as well as the proliferation,of megakaryocytes and may have important therapeutic applications for the treatment of various clinical conditions associated with thrombocytopenia.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人TPO，TPO, Human，Recombinant Human Thrombopoietin
储存条件：冷冻（-20℃）
概述
Thrombopoietin(TPO),the ligand for the receptor encoded by the c-Mpl proto-oncogene,is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo.The cDNAs for TPO have recently been cloned from canine,murine and human sources.The proteins from these three species are highly conserved,exhibiting from 69 - 75%sequence identity at the amino acid level.Two distinct domains,separated by a pair of arginine residues that may be a proteolytic cleavage site,have been identified in TPO:the amino terminal region exhibiting sequence homology to erythropoietin and the carboxy terminal region containing multiple potential N-linked glycosylation sites.Recombinant TPO has now been shown to stimulate the maturation,as well as the proliferation,of megakaryocytes and may have important therapeutic applications for the treatment of various clinical conditions associated with thrombocytopenia.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞98%by SDS-PAGE and HPLC analyses. |
| 来源 | CHO |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human Mo7e cells is less than 3ng/ml,corresponding to a specific activity of>3.3×105 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuTPO a as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH EL |
相关搜索：重组人TPO，TPO, Human，Recombinant Human Thrombopoietin